The "three-into" work of the spirit of the 20th National Congress of the Communist Party of China in colleges and universities is an inevitable requirement to implement the fundamental task of building morality and cultivating people. It is also a practical need to promote ideological and political education in colleges and universities. It is also an urgent need for colleges and universities to cultivate applied talents with good ideological and moral character， strong knowledge ability， deep national identity and high comprehensive quality. Starting from the current situation and problems of the " three-into" work of the spirit of the 20th National Congress of the Communist Party of China in applied undergraduate colleges and universities， this paper optimizes the collaborative education system of the " three-into " work of the spirit of the 20th National Congress of the Communist Party of China， in order to provide some ideas for the application of the "three-into" work of the spirit of the 20th National Congress of the Communist Party of China in application-oriented colleges and universities.